Leader of the research program: Ondřej Novák and the respective department

This research activity is primarily focused on the development of modern, high selective and extremely sensitive analytical methods for the study of physiologically-active low-molecular weight compounds and on the investigation of biological functions of such compounds during the plant growth and development. Targeted and untargeted metabolomic methods will be used for screening of complete metabolome of plant growth regulators and/or monitoring of changes in the metabolism of selected groups. New modern isolation methods and new methods of extraction and purification from complex plant matrices will be developed and implemented. Methods of quantification and identification (fast liquid chromatography and sensitive fast scanning mass spectrometry) will be optimized. The metabolomic approach will contribute to the clarification of the role and function of biologically active compounds in the regulation of developmental processes in plants and in their particular parts (root, shoot, flowers).